Processing of aquatic samples after rinsing/subsampling

Instructions:

1. The sample, found in a plastic jar, should be poured into a large beaker and mixed thoroughly. If the sample is composed of 2 jars, both should be combined and mixed. The sample is then poured into the Subsampling Tray (which resembles the table below, with 7x7 markings for 49 small squares). Generate a unique random number between 1 and 49 using the Generate button, below, to produce the order of taking samples. Prepare vials to hold the following:
   • Worms
   • Mollusks
   • Isopods
   • Other crustaceans
   • Ephemeroptera
   • Odonata
   • Hemiptera
   • Coleoptera
   • Diptera
   • Other
2. Find the corresponding cell on the sieve and remove the contents of the square and spread them out in the narrow counting tray. Use water to help spread the sample in the tray. This step may be done by using parts of the sample in the square if it is particularly dense.
3. Moving from one side to the other, remove all macroinvertebrates from the counting tray. (Microcrustaceans, i.e. copepods, cladocerans, and ostracods, are counted ONLY for the first square of the sample). As removed:
   • Add to the total macroinvertebrate count on the counter.
   • Add to the specific taxon (e.g. physid, planorbid, ancylid, isopod) counter.
   • Place specimen into vial for later identification.
4. Record the running total of macroinvertebrates.
5. Start again at step 2 until the total number of macroinvertebrates equals or exceeds 300. Finish any partially sorted square even if 300 has been reached. Record number of squares sorted.
6. Record counts on data form and store sample jar with remaining sample and vials together.

	A	B	C	D	E	F	G
1	1	2	3	4	5	6	7	7
2	8	9	10	11	12	13	14	14
3	15	16	17	18	19	20	21	21
4	22	23	24	25	26	27	28	28
5	29	30	31	32	33	34	35	35
6	36	37	38	39	40	41	42	42
7	43	44	45	46	47	48	49	49

Generate Reset

Previously selected cells:

Manual Input